![]() ![]() In MCF7 cells, co-immunoprecipitation only occurred with TMEM97 but not with PGRMC1, which was further confirmed by confocal microscopy experiments. Moreover, a close physical colocalization of TMEM97 and TSPO was found in MP cells. Proximity ligation assays and co-immunoprecipitation show a functional association between S2R and TSPO. ![]() Additionally, the role of the reported S2R-interacting partner PGRMC1 was also elucidated. ![]() Here, we investigate the contributions of TMEM97 and TSPO to S2R activity in MCF7 breast adenocarcinoma and MIA PaCa-2 (MP) pancreatic carcinoma cells. Prior to that, we had been investigating the translocator protein (TSPO) as a potential 21.5 kDa S2R candidate protein with reported heme and sterol associations. Recently, S2R was reported to be the transmembrane protein TMEM97. The S2R is highly expressed in various tumors, where it correlates with the proliferative status of the malignant cells. Sigma-2 receptor (S2R) is a S2R ligand-binding site historically associated with reportedly 21.5 kDa proteins that have been linked to several diseases, such as cancer, Alzheimer’s disease, and schizophrenia. ![]()
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